11^th International Conference on Allergy, Asthma & Clinical Immunology September 07-08, 2017 Edinburgh, Scotland

I am Ph.D. in Medical Sciences and assistant professor. I live in Ukraine. I work in the Danylo Halytsky Lviv National Medical University at the Department of Clinical Immunology and Allergology. I work as a doctor of clinical immunologist and allergologist too. I am working towards Molecular Allergology, repeatedly attended courses Molecular school Allergy and Immunology in Vienna and took active part in the work of Ukrainian School of the Molecular Allergology and Immunology, was participant of numerous congresses, conferences, training courses, which are organized by EAACI, valid member of which I am now.

Introduction:The problem of pollen allergy, including pollen of weeds is important for the population of Western Ukraine, including Lviv region. rnrnAim: Compare possibility of SPT and component diagnostics to select appropriate specific immunotherapy.rnrnMaterials and methods: 48 patients of both sexes, aged 18-65 years, residents of Lviv region with seasonal allergic rhinitis / conjunctivitis, selected according to primary stay in the first week of August this year. SPT performed to extracts of pollen allergens from local sources, including a mixture of weeds, grasses and extracts of ambrosia, ragweed, timothy (\"Immunologist\", Ukraine). For molecular researches of sIgE have used ImmunoCAP (Thermo Scientific, Uppsala, Sweden).rnrnResults: In 50% of patients found positive SPT to mixture of weed, extracts of ambrosia, ragweed and grass mixtures. This indicated to co-sensitization to various sources of allergens. 30% of patients had monosensitization to weeds pollen, and 20% - monosensitization to grass pollen. However, simultaneous sensitization to pollen of ambrosia, ragweed and timothy has not been proven by molecular researches. Instead, 20% of patients identified sensitization to ragweed and ambrosia, 30% of people - monosensitization to ambrosia, and 20% - monosensitization to ragweed. Most (70%) of patients with monosensitization to pollen of weeds identified specific IgE to Art v1 and/or Art v3, and / or Amb a1. False positive results of SPT indicated that co-sensitization to grasses and weeds can be explained by the presence of sIgE for cross-reactive markers of profilin Phl p 12 and polcalcin - Phl p 7. rnrnConclusion: On the basis of SPT and molecular researches doctor takes a fundamentally different decision on the selection of extracts for specific allergen immunotherapy. Optimal allergic immunotherapy based on the identification of primary sensitizer and cross-reactivity markers. rnrnKey words: weeds, component diagnostic, primary sensitizer, cross-reactivity, allergen immunotherapy.rn

My name is Marta Lomikovska, from 1996 to 2002. I studied at the medical faculty of Danylo Halytskyy Lviv National Medical University, successfully passed exams and I got qualification Doctor. After finishing University from 2002 to 2003 I studied in the internship on speciality \"Тherapy\" in Lviv National Medical University and Lviv Regional Clinical Hospital. From 2003 to 2008 worked at the Sokilnyky Medical Clinic as a therapist. From 2011 till 2013 I worked at the Clinical Immunology and Allergology Department as a senior laboratory in Lviv National Medical University. From 2014 and till now I am working as assistant professor at the same Department. I am an active participant of national congresses and conferences with international participation, which regularly held in Ukraine, Spain and Austria. I am an author of 15 scientific publications in national and international journals in this area of work.

Relevance: The problem of reactive arthritis (ReA) is related to its prevalence, diagnostic difficulties, involvement of many organs and systems in the pathological process, insufficiently effective treatment. \r\n\r\nObjective: To study the clinical and immunological features of the course and diagnostics of ReA against the background of Epstein-Barr virus infection.\r\n\r\nMaterials and methods: 24 patients with clinical manifestations of arthritis have been examined. General laboratory, immunological, serological and molecular genetic studies to determine EBV-infection has been conducted for the patients. \r\n\r\nResults and discussion: Clinically, all patients experienced arthralgic. In addition, 6 (25%) people have been diagnosed with long-lasting subfertility, 16 (66.67%) - chronic fatigue syndrome, 7 (29.1%) - respiratory immunodeficiency. According to the research results 5 (20.8%) patients had positive rheumatoid factor, 6 (25.0%) - increased ESR, 10 (41.7%) - increased AS(L)O level, and 5 (20.8%) - increased concentrations of CRP. High titers of specific antibodies of class IgM, IgG to capsid and nuclear antigens ЕВV and ЕВV-DNA have been found in saliva, mucous membrane scraping and blood of the patients by the method of polymerase chain reaction. The immunogram analysis more often pointed to the presence of immunodeficiency by the combined lymphocytic-phagocytic type in 18 (75%) of the examined, among whom lymphocytosis was observed in 7 (29.1%) patients. What is more, the increased number of natural killer cells was determined in 9 (37.5%) patients, and in 13 (54.1%) - changes of CD8+-lymphocytes. 13 (40.6%) patients had increased levels of T-helper cells, which can be interpreted as a prerequisite for the formation of auto aggression. Patients were treated with antiviral, immunotropic, symptomatic therapy, specific immunoglobulins.\r\n\r\nConclusions: ReA of EBV-origin was most often found in women (70.8%) aged 18-35, and was characterized by an increase rheumatoid factor (20.8%), CRP (20.8%), AS(L)O (41.7%), ESR (25%) against the background of the absence of autoantibodies specific for rheumatoid arthritis.\r\n